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lympholyte®-h cell separation density gradient solution  (Cedarlane)


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    Cedarlane lympholyte®-h cell separation density gradient solution
    Lympholyte® H Cell Separation Density Gradient Solution, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/density+gradient+separation+solution/lympholyte+m+gradient/pm40164596-49-4-10
    Average 90 stars, based on 1 article reviews
    lympholyte®-h cell separation density gradient solution - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics
    Article Snippet: PB mononuclear cells (PBMCs) were isolated from buffy coats of HDs ( n = 6 ) or from six healthy vaccinated subjects through Lympholyte ® -H Cell Separation density gradient solution (Cedarlane Laboratories, Burlington, North Carolina, USA) according to the manufacturer’s instruction.

    Article Title: Immune evasion mechanisms in early-stage I high-grade serous ovarian carcinoma: insights into regulatory T cell dynamics.
    Article Snippet: PBMCs were isolated using Lympholyte®-H Cell Separation density gradient solution (Cedarlane Laboratories, USA) according to the manufacturer’s instructions and were frozen.

    Article Title: Transcriptomic profile of TNF high MAIT cells is linked to B cell response following SARS-CoV-2 vaccination
    Article Snippet: Freshly PBMCs were isolated through Lympholyte ® -H Cell Separation density gradient solution (Cedarlane Laboratories, Burlington, North Carolina, USA) according to the manufacturer’s instructions and frozen in Fetal Bovine Serum (FBS, EuroClone) supplemented with 10% of the cryoprotective dimethyl sulfoxide (DMSO, PanReac AppliChem) as previously published ( ).

    Isolation:

    Article Title: Identification of Tissue-Resident Natural Killer and T Lymphocytes with Anti-Tumor Properties in Ascites of Ovarian Cancer Patients.
    Article Snippet: At the end, the single-cell suspension was frozen in fetal bovine serum (FBS; Lonza, Basel, Switzerland) supplemented with 10% of dimethylsulfoxide (DMSO; Lonza) in liquid nitrogen for further analysis. .. The peripheral blood mononuclear cells (PBMCs) were isolated from freshly isolated blood of HGSC patients using a Lympholyte® Cell Separation density gradient solution (Cederlane Laboratories: Burlington, ON, Canada) following the manufacturer’s instructions. ..

    Article Title: Intrahepatic CD69 + Vδ1 T cells re-circulate in the blood of patients with metastatic colorectal cancer and limit tumor progression
    Article Snippet: .. Peripheral blood mononuclear cells obtained from the buffy coats of healthy donors (HDs) or from blood of patients with CLM were isolated by Lympholyte Cell Separation density gradient solution (Cederlane Laboratories, Burlington, Canada) according to the manufacturer’s instructions. .. Liver specimens were dissociated by enzymatic digestion with 2 mg/mL of collagenase D (Roche Diagnostic; Indianapolis, Indiana, USA) in gentleMACS Dissociator (Miltenyi; Bergisch Gladbach, Germany) as previously described.

    Article Title: NKG2A expression identifies a subset of human Vδ2 T cells exerting the highest antitumor effector functions.
    Article Snippet: .. Human specimens and cell culture Peripheral bloodmononuclear cells (PBMCs) were isolated from buffy coats of HDs or from freshly isolated blood of patients affected byHCC, GBMandNSCLC through Lympholyte Cell Separation density gradient solution (Cederlane Laboratories; Burlington, Canada) according to the manufacturer’s instruction. .. Cell Reports 37, 109871, October 19, 2021 e3 Freshly isolated HCC liver tissue was dissociated by enzymatic digestion in gentleMACSDissociator (Miltenyi; Bergisch Gladbach, Germany) with 2 mg/mL of collagenase D (Sigma Aldrich; St. Louis, Missouri, USA) for 45 minutes at 37 C/5% CO2.

    Article Title: Distinct responses of newly identified monocyte subsets to advanced gastrointestinal cancer and COVID-19
    Article Snippet: .. Samples were collected in EDTA-coated tubes (BD Vacutainer K2E), and peripheral blood mononuclear cells (PBMCs) were isolated by Lympholyte ® cell separation density gradient solution (Cederlane). .. Any residual erythrocytes were removed via ammonium-chloride-potassium (ACK) Lysing Buffer (Lonza) treatment for 60 sec at room temperature (RT).

    Article Title: Identification of Tissue-Resident Natural Killer and T Lymphocytes with Anti-Tumor Properties in Ascites of Ovarian Cancer Patients
    Article Snippet: At the end, the single-cell suspension was frozen in fetal bovine serum (FBS; Lonza, Basel, Switzerland) supplemented with 10% of dimethylsulfoxide (DMSO; Lonza) in liquid nitrogen for further analysis. .. The peripheral blood mononuclear cells (PBMCs) were isolated from freshly isolated blood of HGSC patients using a Lympholyte ® Cell Separation density gradient solution (Cederlane Laboratories: Burlington, ON, Canada) following the manufacturer’s instructions. ..

    Cell Culture:

    Article Title: NKG2A expression identifies a subset of human Vδ2 T cells exerting the highest antitumor effector functions.
    Article Snippet: .. Human specimens and cell culture Peripheral bloodmononuclear cells (PBMCs) were isolated from buffy coats of HDs or from freshly isolated blood of patients affected byHCC, GBMandNSCLC through Lympholyte Cell Separation density gradient solution (Cederlane Laboratories; Burlington, Canada) according to the manufacturer’s instruction. .. Cell Reports 37, 109871, October 19, 2021 e3 Freshly isolated HCC liver tissue was dissociated by enzymatic digestion in gentleMACSDissociator (Miltenyi; Bergisch Gladbach, Germany) with 2 mg/mL of collagenase D (Sigma Aldrich; St. Louis, Missouri, USA) for 45 minutes at 37 C/5% CO2.



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